RESUMO
A BACTERIOLOGICAL STUDY OF URINARY TRACT INFECTION INVOLVING CULTURAL ANALYSIS Original Research Paper Estibeiro AS* Associate Professor, Department of Microbiology, Goa Medical College, Bambolim Goa, 403202 *Corresponding Author Background: Urinary Tract Infections are a common occurrence in the community and hospital and are associated with profound morbidity and decreased productivity at work. Timely diagnosis of UTI by cultural analysis is imperative in order to identify the offending agent and to initiate appropriate antimicrobial therapy. Objectives: The present study aimed at evaluating the bacteriological pro?le of UTI employing semiquantitative culture and to determine the antibiogram pattern of the bacteria isolated. The study was conducted on 200 urine samples, collected Methods: from clinically suspected cases of UTI. Semiquantitative analysis was done and antimicrobial sensitivity testing undertaken, as per CLSI guidelines. Signi?cant bacteriuria was observed in 66.5% cases. Gram negative bacilli were Results: predominant pathogens (71.3%), amongst which, Escherichia coli and Klebsiella pneumoniae were the main agents (37.2% and 24.5 % respectively). Gram positive organisms accounted for 28.7% of the total; Enterococcus species being the predominant isolate type (78.04%; 32 out of 41 gram positive pathogens). Overall, Escherichia coli predominated (26.6%), followed by Enterococcus species (22.4%). Gram negative bacilli showed good sensitivity to Imipenem and Aminoglycosides. Gram positive cocci were all sensitive to Vancomycin and Linezolid. A large number of cases were 50 years and above (51%), followed by age groups 21-30 years (17.5%) and 31 to 40 years (16.5%). The Male: Female ratio was 1.2:1, males accounting for 53.5% of the total. Early cultural analysis of UTI cases with identi?cation of the etiological agent and its Conclusion: antibiogram pattern will help in early treatment and use of appropriate antibiotics. This will reduce the use of empirical treatment, morbidity and chronicity